ENCODED - significado y definición. Qué es ENCODED
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Qué (quién) es ENCODED - definición

METHOD TO REPRESENT INFORMATION FOR VARIOUS PURPOSES (STORAGE, TRANSMISSION, PROTECTION AGAINST UNAUTHORIZED ACCESS ETC.)
CodE; Encoding; Encoding code; Encode/Decode; Codes; Encoded; Code (communications); Code (information theory); Coding and decoding; Encodings; Information encoding; Código; Data encoding; CØDE

DNA-encoded chemical library         
  • '''Fig. 2''' '''DNA-encoded library by ‘DNA-templated synthesis’'''A library of oligonucleotides (i.e. 64 different oligonucleotides) containing three coding regions was hybridized to a library of reagent compound-oligonucleotide conjugates (i.e. 4 reagent oligonucleotide conjugates), able of pairing with the initial coding domain of the template oligonucleotide. After transferring of the compounds on the corresponding oligonucleotide template, the synthesis cycle was repeated the desired number of times with further sets of carrier compound-oligonucleotide conjugates (i.e. two rounds with four carrier compound-oligonucleotide conjugates per round). Subsequently, functional selection was performed and the sequence of the binding template amplified by PCR. Thus, DNA-sequencing allowed the identification of the binding molecule.
  • '''Fig. 1''' '''DNA-encoded library displaying chemical compounds''' Schematic representation of DNA-encoded library displaying chemical compounds directly attached to oligonucleotides. a) Library generated by “stepwise combinatorial” assembling presenting a single oligonucleotide covalently linked to a putative binding molecule. b) Library construct in “combinatorial self-assembling” fashion ('''E'''ncoded '''S'''elf-'''A'''ssembling '''C'''hemical library). Multiple pairing oligonucleotides display a covalently linked binding molecule
  • '''Fig. 3''' '''DNA-encoded library by "Split-&-Pool stepwise coupling of coding DNA fragments to nascent organic molecules''' An initial set of multifunctional building blocks (FGn represents the different orthogonal functional groups) are covalently conjugated to a corresponding encoding oligonucleotide and reacted in a split-&-pool fashion on a specific functional group (FG1 in red) with a suitable collection of reagents. Following enzymatic encoding, a further round of split-&-pool is initiated. At this stage the second functional group (FG2 in blue) undergoes an additional reaction step with a different set of suitable reagents. The identity of the final modification could be ensured yet again by enzymatic DNA encoding by means of a further oligonucleotide carrying a specific coding region.
  • '''Fig. 4''' '''ESAC library technology overview''' Small organic molecules are coupled to 5’-amino modified oligonucleotides, containing a hybridization domain and a unique coding sequence, which ensure the identity of the coupled molecule. The ESAC library can be used in single pharmacophore format (a), in affinity maturations of known binders (b), or in de novo selections of binding molecules by self assembling of sublibraries in DNA-double strand format (c) as well as in DNA-triplexes (d). The ESAC library in the selected format is used in a selection and read-out procedure (e). Following incubation of the library (i) with the target protein of choice (ii) and washing of unbound molecules (iii), the oligonucleotide codes of the binding compounds are PCR-amplified and compared with the library without selection on oligonucleotide micro-arrays (iv, v). Identified binders/binding pairs are validated after conjugation (if appropriate) to suitable scaffolds (vi).
  • '''Table 1. ''' '''YoctoReactor library size.''' yR library size is a function of the number of different functionalized oligos used in each position and the number of positions in the DNA junction
  • '''Fig. 5''' '''Fundamental principle of the YoctoReactor.''' The center of 3, 4 and 5 way DNA junctions (a 4-way junction is shown here) becomes a [[yoctoliter]]-scale reactor where small molecule synthesis is facilitated in what has been termed the YoctoReactor (yR). Colored circles depict the chemical building blocks (BB) which are attached to carefully designed DNA oligonucleotides (black lines). Upon DNA annealing the BB are brought into proximity at the center of the DNA junction where they undergo chemical reaction.
  • '''Fig. 6''' '''YoctoReactor library assembly.''' Stepwise assembly of a DEL library using YoctoReactor technology. A 3-way reactor is shown here. (a) Position 1 (P1) and P2 BB are brought into proximity and undergo a chemical reaction in the presence of a helper oligonucleotide in P3. (b) The structure is purified by polyacrylamide gel electrophoresis (PAGE), the P1 and P2 DNA is ligated and the P2 linker is cleaved. (c) P3 BB is annealed to the P1-P2 ligation product from step b, and a chemical reaction between P2 and P3 BBs takes place. (d) The reaction product is purified by PAGE, the DNA is ligated and P3 linker is cleaved yielding a compound (OOO) covalently attached to the folded yR. (e) The yR is dismantled by primer extension yielding a double-stranded display product exposing the reaction product for selection and molecular evolution.
User:Martina Steiner/DNA Encoded Chemical Libraries; DNA Encoded Chemical Libraries; DNA Encoded Chemical Library
DNA-encoded chemical libraries (DEL) is a technology for the synthesis and screening on unprecedented scale of collections of small molecule compounds. DEL is used in medicinal chemistry to bridge the fields of combinatorial chemistry and molecular biology.
Serial time-encoded amplified microscopy         
  • Illustration of warped stretch transform in imaging.
  • Time stretch quantitative phase imaging system is an artificial intelligence facilitated microscope that includes a big data analytics pipeline for machine vision and learning. [http://www.nature.com/srep/about/open-access Image licensing CC BY 4.0] -
OPTICAL IMAGING METHOD
Serial time encoded amplified microscopy (STEAM); Serial time-encoded amplified microscopy (STEAM); Stretched Time Encoded Amplified Imaging/Microscop; Time stretch quantitative phase imaging
Time Stretch Microscopy also known as Serial time-encoded amplified imaging/microscopy or stretched time-encoded amplified imaging/microscopy' (STEAM) is a fast real-time optical imaging method that provides MHz frame rate, ~100 ps shutter speed, and ~30 dB (× 1000) optical image gain. Based on the Photonic Time Stretch technique, STEAM holds world records for shutter speed and frame rate in continuous real-time imaging.
CODE         
Client/server Open Development Environment (Reference: Powersoft)

Wikipedia

Code

In communications and information processing, code is a system of rules to convert information—such as a letter, word, sound, image, or gesture—into another form, sometimes shortened or secret, for communication through a communication channel or storage in a storage medium. An early example is an invention of language, which enabled a person, through speech, to communicate what they thought, saw, heard, or felt to others. But speech limits the range of communication to the distance a voice can carry and limits the audience to those present when the speech is uttered. The invention of writing, which converted spoken language into visual symbols, extended the range of communication across space and time.

The process of encoding converts information from a source into symbols for communication or storage. Decoding is the reverse process, converting code symbols back into a form that the recipient understands, such as English or/and Spanish.

One reason for coding is to enable communication in places where ordinary plain language, spoken or written, is difficult or impossible. For example, semaphore, where the configuration of flags held by a signaler or the arms of a semaphore tower encodes parts of the message, typically individual letters, and numbers. Another person standing a great distance away can interpret the flags and reproduce the words sent.

Ejemplos de uso de ENCODED
1. Encoded on the passport’s RFID chip are three important files.
2. Who wants DRM crippled rubbish encoded MP3‘s anyway?
3. Unlike other internet voice services, Skype calls are encrypted – encoded using complex mathematical operations.
4. But it was the distinct horror of his sergeant‘s death that was encoded in his brain.
5. That‘s not a coincidence; it was encoded deep in the creed of Blairism.